Carine satge

Arrival date : 2017

Status : Postdoctoral

Roles :

- Development of technologies for capturing large DNA fragments in order to facilitate the analysis of agrnomic interest regions in plants

Last completed degree : PhD in Plant Biotechnologies

Professional experiences :

- 2013-2016 : Thesis at the Laboratory of Plant-Microbe Interactions, Toulouse, France - Study of epigenetic mechanisms involved in the nodule development during the symbiosis between the legume Medicago truncatula and its bacterial host Sinorhizobium meliloti


    CATCHMI

    CATCH My Interest : capture of large genomic regions of interest

    The CATCHMI project aims at developing a new approach to capture specific genomic regions of interest.

    Added on : 30 August 2017

    Read more

    Project coordinator:

    CNRGV
    Hélène BERGES

     

    Project partners:

    INRA - LIPM
    Stéphane Munos
    Nicolas Langlade
    24 chemin de Borde Rouge
    31326 Castanet Tolosan
    Email : stephane.munos@inra.fr

    LAAS - CNRS
    Aurélien Bancaud
    7, avenue du colonnel Roche
    31077 Toulouse
    Email : abancaud@laas.fr

     

    Abstract:

    Agricultural research must deal with major issues on various scales, in light of the changing climatic and demographic context, where energy resources are limited. In this context of a need for improvement and adaptation of plants, genomic exploration is one of the strategic approaches of choice. Indeed, genomics will allow to define the gene content, their organization, their biological function and their variability between the different varieties. This knowledge facilitate the identification of interesting plant genes, which can play a role in biotic or abiotic resistance, in yield or in the quality process. However, the exploration of plant genome can be challenging  due to the complexity of plant genomes in terms of size, repetitive elements content and various levels of ploidy.
    Linking a phenotype to a genomic region is crucial to better understand biological process. However, these approaches are still based on the study of the whole genome. Most research projects require a reliable  sequence of the region of interest genetically characterized or to be able to explore these regions on a larger population of individuals.
    To meet these expectations, the CATCHMI project aims at developing a new approach to capture specific genomic regions of interest. This innovative strategy is based on the CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) technology. Indeed, this technique used in vitro can be an effective means of targeting, excising and characterizing a specific region of a genome. This CRISPR-CATCH method was tested on a bacterial genome  (Jiang et al., 2015). We'll develop this approach on large and complex plant genome regions in Sunflower.

     

    CNRGV's responsible

    • Carine Satgé

     

    CNRGV involvement

    • Development of this new approach.

     

     

    Fund agency: 

    This project is funding by the Plant2Pro Carnot Institute. This institute is dedicated to integrated R&D “from laboratory to field” in the area of agricultural crop production.

    http://www.instituts-carnot.eu/en/carnot-institute/plant2pro

    institut-carnot plant2pro

    YouTube : https://youtu.be/PWy7SZl0MW8