Towards the understanding of the mechanisms involved in frost tolerance in pea.

In collaboration with :

Bruno Delbreil
UMR USTL INRA 1281, Stress abiotiques et différenciation des végétaux cultivés
Université des Sciences et technologies de Lille, Bâtiment SN2
59650 Villeneuve d’Ascq
France
Phone: +33 320434022
E-mail: bruno.delbreil@univ-lille1.fr

Isabelle Lejeune
UMR USTL INRA 1281, Stress abiotiques et différenciation des végétaux cultivés
80200 Estrées-Mons
France
Phone: +33 322857507
E-mail: Lejeune@mons.inra.fr

Nasser Bahrman
UMR USTL INRA 1281, Stress abiotiques et différenciation des végétaux cultivés
Université des Sciences et technologies de Lille, Bâtiment SN2
59650 Villeneuve d’Ascq
France
Phone: +33 322857854
E-mail: nasser.bahrman@mons.inra.fr

Abstract :

Dry pea is an important field crop in France. The sowing of dry pea in autumn allows a longer life cycle than in a spring sowing, associated with a higher biomass production that would help to improve and stabilize this crop productivity. However, to release winter varieties, winter survival has still to be improved and particularly the freezing tolerance component. On pea, the genetic determinism of frost tolerance has been studied. Three major QTLs have been mapped on linkage groups 3, 5 and 6.

Pea BAC-library are important genomic tools suitable for physical mapping and map-based cloning. This library is being screened with probes corresponding to markers in the QTL interval. The physical map of the corresponding region is under construction and BACs covering the reduced confidence interval of the QTL will be sequenced. This approach that combine fine mapping and positional cloning will lead us to identify potential candidate genes underlying the three freezing tolerance pea QTLs. 

Recent publications :

E. Dumont, N. Bahrman, E. Goulas, B. Valot, H. Sellier, JL. Hilbert, C. Vuylsteker, I. Lejeune-Hénaut, B. Delbreil. A proteomic approach to decipher chilling response from cold acclimation in pea (Pisum sativum L.)  Plant Sci. 2011 (180-1) pp 86-98

E. Dumont, V. Fontaine, C. Vuylsteker, H. Sellier, S. Bodèle, N.Voedts, R.Devaux, M.Frise, K. Avia, JL Hilbert, N.Bahrman, E.Hanocq, I.Lejeune-Hénaut, B.Delbreil. Association of sugar content QTL and PQL with physiological traits relevant to frost damage resistance in pea under field and controlled conditions. TAG 2008 (118-8) pp 1561-1571

CNRGV's responsible: Sonia Vautrin