ZeaWall : Towards the identification of genetic determinisms involved in lignified secondary cell wall degradability through the elucidation of QTLs
Project coordinators :
Le Chêne - RD 150
Email : Yves.Barriere@lusignan.inra.fr
Route de Saint Cyr - RD10
78026 Versailles cedex
Email : Matthieu.Reymond@versailles.inra.fr
Project partners :
ProMaïs Crop companies (Website: http://pro-mais.org)
24 Chemin de Borde Rouge
31326 Castanet Tolosan
Email : firstname.lastname@example.org
UMR de Génétique Végétale
Ferme du Moulon
91190 Gif-sur-Yvette, France
Email : email@example.com
Highlighting the genetic determinants of the lignified cell wall assembly in grasses is a major challenge for both basic research, because of the specific parietal traits of these species, and for plant breeding based on marker-assisted selection. Cell wall degradability is a limiting factor of plant energy value for cattle feeding, as well as for the production of second-generation biofuel. The research conducted, in collaboration between INRA and ProMaïs, thus aims at identifying genetic determinisms involved in cell wall related traits, taking as a model a cluster of strong effect QTLs located in a chromosome 6 genomic region of the maize recombinant inbred line (RIL) F288 x F271.
In order to characterize the region responsible for these traits, targeted sequencing of this genomic region was undertaken in the two parental lines F288 and F271 through a BAC library approach.
Courtial A, Méchin V, Reymond M, Grima-Pettenati J, Barrière Y, 2013. Colocalizations between several QTLs for cell wall degradability and composition in the F288 x F271 early maize RIL progeny raise the question of the nature of the possible underlying determinants and breeding targets for biofuel capacity. BioEnergy Research 7(1):142-156.
Courtial A, Thomas J, Reymond M, Méchin V, Grima-Pettenati J, Barrière Y, 2013. Targeted linkage map densification to improve cell wall related QTL detection and interpretation in maize. Theor Appl Genet 126: 1151-1165.
Responsible: Audrey Courtial
BAC libraries construction and screening for F288 and F271 lines
BAC sequencing and comparative analysis