luiz

Luiz Augusto Cauz dos Santos

Luiz Augusto Cauz dos Santos

luizcauz@usp.br

Date d'arrivée : 18/06/2019

Date de départ : 18/12/2019

Statut : Stagiaire

Rôles :

Luiz est de retour pendant 6 mois @CNRGV pour mener à bien son projet de thèse visant à explorer la diversité de la passiflore en séquençant les génomes chloroplastiques de variétés sauvages</span>

Dernier diplôme obtenu : Master en Biotechnologies (UFGD / Brazil)

Expériences professionnelles :

2014-_aujourd'hui: Thèse en Génétique et amélioration des plantes - University of Sao Paulo (USP), Brazil.06-2018 – 09/2018: Développement d’un protocole d’isolement d’ADN chloroplastique sur différentes espèces de Passiflore -CNRGV-France2012 _2013: formation de premier cycle en génétique - University of Barcelona (UB).


    passiflore projet luiz

    Phylogenetic inferences in Passiflora based on the analysis of chloroplast genomes

    Using chloroplast genome sequences (cpDNA), our research group has carried out phylogenomic analyses that resulted in strongly supported trees, providing a better understanding of the evolutionary relationships in the Malpighiales and the Fabids' clade...

    Ajouté le : 05 septembre 2018

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    The genus Passiflora consists of some 520 species, 145 of which occur in Brazil, and 83 are endemic to the country. The classical taxonomy, proposed on the basis of floral and vegetative structures, subdivided the genus into 22 subgenera. However, molecular phylogenetic analyses did not confirm that infrageneric subdivision, reducing the number of subgenera to four: Astrophea, Decaloba, Deidamioides and Passiflora, being Astrophea the more ancestral subgenus. Three clades were recognized as monophyletic (Astrophea, Decaloba, and Passiflora), but the position of Deidamioides remained unresolved, as this particular clade was found to be paraphyletic based on current data. Using chloroplast genome sequences (cpDNA), our research group has carried out phylogenomic analyses that resulted in strongly supported trees, providing a better understanding of the evolutionary relationships in the Malpighiales and the Fabids' clade. This analysis included the cpDNA of Passiflora edulis. In this context, the same approach will be applied to perform an evolutionary study in Passiflora, aiming to help to resolve the question about the status of the subgenus Deidamioides. The cpDNA of P. edulis revealed rearrangements that led to changes in the order of some genes. We suggest that these rearrangements may be associated to the infrageneric division. Moreover, the genes rpl22 and accD have been lost in P. edulis cpDNA and possibly were exported to the nucleus. We will investigate this finding, aiming to verify our hypothesis. The cp genome annotation is a crucial step, due to the presence of inverted repeat sequences and rearrangements. Therefore, we propose to use long sequence reads for generating accurate biological information to be use in the phylogenetic analyses. In this project we will perform field work aiming to collect 50 species of Passiflora, building two specimen-vouchered DNA banks: cpDNA and total DNA, and a selection of these will have their plastomes sequenced to answer the questions in a phylogenomic and evolutionary context.

     

    Collaborators:

     - Dra. Maria Lucia Carneiro Vieira (Main responsible)

    Escola Superior de Agricultura "Luiz de Queiroz" /Universidade de São Paulo

    Genetics Department

    Avenida Pádua Dias, nº11 – Agronomia 

    13400-970 Piracicaba, Brasil

    Phone: 55 19 3429 4442

    -  Dr. Cássio Van den Berg

    Universidade Estadual de Feira de Santana/

    Bahia ,Brazil

     

    Publications:

    Cauz Santos LA, Freitas Munhoz C, Rodde N, Cauet S, Azevedo Santos A, Alves Penha H, Carnier Dornelas M, de Mello Varani A, Conde Xavier Oliveira G, Bergès H, Carneiro Vieira ML (2017) The Chloroplaste Genome of Passiflora edulis (Passifloraceae) Assembled from Long Sequence Reads: Structural Organization and Phylogenomic Studies in Malpighiales- Front. Plant Sci. 8:334. DOI: 10.3389/fpls.2017.00334

     

    Responsible CNRGV: Caroline Callot