Adaptive Sampling by Oxford Nanopore Technologies: capture by selective sampling

The CNRGV is developing advanced methods for targeted sequencing, leveraging the Oxford Nanopore Technologies P2 sequencer's capability to selectively sequence specific genome fractions. This approach, known as Nanopore Adaptive Sampling (NAS), uses real-time comparison between DNA molecules being sequenced and a database of target sequences. Based on predefined criteria, each DNA molecule is either sequenced or rejected, enabling highly selective data production.

NAS facilitates the sequencing of specific genomic regions (e.g., exons, genes, or entire chromosomes) without requiring physical enrichment protocols or additional reagents. It also allows for selective sequencing within complex samples, such as DNA from plant-microbiota interactions. For instance, bacterial DNA can be sequenced exclusively by excluding plant DNA.

The CNRGV is actively developing several methods to expand NAS-based services. If you are interested in NAS, we encourage you to contact us to discuss the feasibility of your project or to explore potential collaborations for method development.

Ongoing tests are currently funded by the Carnot Plant2Pro institute in the framework of the GREASE project.